From 1998 Constantin Frerick studied Chemical Engineering at TU Dortmund University. In November 2003 he graduated with the diploma thesis "Rigorous Modelling of Reactive Desorption in an Amine Scrubber". Since March 2004 he was employed as scientific assistant at the laboratory of fluid separations at TU Dortmund University. In July 2009 he graduated with the PhD thesis "Rigorous Modelling, Simulation and Evolutionary Optimisation of Generic Protein Downstream Processes". Since March 2008 he is working for Bayer Technology Services GmbH in the competence center Process Analysis.
Member of research staff at the laboratory of fluid separations until December 2007 in the field of purification of biotechnological products.
Rigorous Modelling, Simulation and Evolutionary Optimisation of Generic Protein Downstream Processes
I worked on a generic process model for the simulation of various downstream processes for protein purification. It consists of detailed, dynamic models for ion-exchange membrane adsorbers, ion-exchange and size-exclusion chromatography and ultra-/diafiltration. For the implemented ion-exchange membrane adsorber model, the steric mass action model has been applied. The isotherm parameters have been determined experimentally for bovine serum albumin (BSA) and immunoglobulin G (IgG). The comparison between simulation results of the ion-exchange membrane and experiments for two protein mixtures containing BSA and IgG showed satisfactory agreement. Two case studies based on the purification process of human serum albumin were performed to illustrate the influence of structural changes in the process flowsheet on the process performance. The simulation results showed that the replacement of an anion-exchange chromatography column by an anion-exchange membrane adsorber leads to a significant reduction in overall process time at constant yield and purity. The case studies demonstrated the applicability of the generic process model for detailed process analysis.
|"Rigorous Modelling, Simulation and Evolutionary Optimisation of Generic Protein Downstream Processes"
|Dr. Hut (2009)